ISOLATION AND SIGNIFICANCE OF AEROMONAS HYDROPHILA GROUP IN FARMED RABBITS AT ASSIUT GOVERNORATE عزل وأهمية مجموعة الأيروموناس هيدروفيلا فى أرانب المزرعة فى محافظة أسيوط

Document Type : Original Article

Abstract

ABSTRACT :




A total of 135 faecal swabs were aseptically collected from different rabbit farms at Assiut Governorate. Diarrhea and emaciation were observed in 120 out of 135 while the rest were apparently healthy.. These samples were examined bacteriologically for determination of the occurrence and frequency of Aeromonas hydrophila. The obtained results revealed that total isolates of A.hydrophila were 35 at percentage of (25.9%) which represented 33 isolates from diarrhotic rabbits at percentage of (24.4%) while 2 isolates were obtained from apparently healthy animals at percentage of (1.5%). 
The experimental infection in 6-8 week-old rabbits by oral route led to 20% mortality. The clinical observation and the post-mortem lesions of experimentally infected animals were recorded Clinical observations were similar to a great extent to those of natural infection. Reisolation of infecting organism from internal organs and intestinal tract of dead and scarified slaughtered rabbits at the end of observation period were conducted.
The in vitro susceptibility of the A.hydrophila isolates to a variety of antibiotics revealed that highest number of isolates were sensitive to Gentamyicin (100%), Nalidxic acid (100%), Chlormphnicol (95%) and Cephoxetin (90%), while it was resistant to Penicillin and Ampicillin.
The public health significance and the economic losses arising from infection of the rabbit with A.hydrophila as well as suggestions for their avoidance were discussed.
تم عمل استبيان لدراسة مدى انتشار عدوى ميکروب الأيروموناس هيدروفيلا فى مزارع الأرانب فى محافظة أسيوط  أجريت الدراسة على135 عينة براز من مزارع الأرانب  شملت على 120 حالة تعانى من الإسهال والضعف العام  و15 حالة سليمة ظاهريا وتم فحص العينات بکتريولوجيا لاستبيان مدى تواجد ميکروب الأيروموناس هيدروفيلا فى أرانب المزارع  وعلاقتها بالإسهال وکانت نتائج الفحص البکتريولوجى تشير إلى عزل 35 عترة من الميکروب من جميع الحالات بنسبة 25.4% منها 24.4% من حالات الأسهال و1.5% من الحالات السليمة ظاهريا و بإجراء العدوى الصناعية بهذا الميکروب فى الأرانب عمر 6-8 أسابيع بواسطة الحقن عن طريق الفم وجدت أنها ضارية للأرانب حيث بلغت نسبة النفوق إلى 20%، وقد سجلت  الأعراض الإکلينيکية والآفات التشريحية ووجد آن الأعراض الأکليکينية  تشبه إلى حد کبير تلک التى لوحظت فى العدوى الطبيعية0 هذا وقد تم عزل الميکروب مرة أخرى من الأعضاء الداخلية والأمعاء من الأرانب النافقة والمصابة0 کما تم عمل اختبار الحساسية للميکروب المعزول وکان شديد الحساسية لکل من الجنتاميسين والنلادکس اسيد والکولورمنيفينکول والکاناميسين بينما کان مقاوم لکل من البنسلين والامبسلين0 وتم مناقشة الأهمية الصحية من تواجد هذا الميکروب وعلاقته بحالات الإسهال فى الأرانب

References

REFERENCES:
Ali, A.M.; EL. Sanousi, S.M.; AL-Eknah.M.A.; Gameel,A.A.; Dafalla,E.A.; Homeid, A.M. and Radwan,Y.M. (1992): Studies on infundibular cyst of the uterine tube in camel. Revu. Med. Vet. Pays Trop. 45: 4,253.
Alonso, J.M.; Rey,J.M.; Hermoso DE, J. and Hermoso de Mendoza, M. (1996): Aeromonas hydrophila: an unusual case of pneumonia in goats. Medicine Veterinary, 13: 439-441.
Bisgaard,M.; Bianucci, F.and Sacchetti, R. (1995): Prevalence of Aeromonas spp. In surface waters Wal. Environ. Res.,67 (7), 1060-1064.
Bullock, G.L.; Conory,D.A. and Snieszko, S.F. (1971): Bacterial diseases of fishes. pp.1, 285. T.F.H publication, Inc.Neptune City, N.J.
Davis, W.A., I.J. Kane and V.F. Garagusi (1978): Human Aeromonas infections a review of the literature and a case report of endocarditis. Medicin (Baltinore). 57: 267-277.
Efuntoye, M.O. (1995): Diarrhoea disease in livestock associated with Aeromonas hydrophila biotype1. J.Gen.Appl. Microbiol, 41:(6) 517-521.
Elliot, D. and Shotts, E.Jr (1980): Etiology of an ulcerative disease in goldfish. Carassius auratus (L): Experimental induction of the disease. J. Fish Dis., 3: 687-693.
Fass, R.J. and J. Barnishan (1981) In Vitro susceptibility of Aeromonas hydrophila to 32 antibiotics. Antimicrob. Agents Chemother. 19: 357-358.
Finegold,S.M. and Martin, W.J. (1982): Cited after Bailly and Scotts, Diagnosis Microbiology, 16th Ed. Th C.V. Mosby Co.St. Louis Toronto London.
Glunder, G. and Siegmann, O. (1989): Occurrence of Aeromonas hydrophila in slid birds. Avian pathol. 18, 685-695.
Hazen, T.C., C.B., Fliermans; R.P. Hirsch and G.W.Esch (1978): Prevalence and distribution of Aeromonas hydrophila in USA J.App. Environ.Microbiol 36, (5) : 731-738.
Hazen, T.C.; Racker,M.L.; Esch,G.W. and Fliermans, C.B. (1978): Ultra-structure of red- sore lesion on largemouth (Micropterus salmoidsh) association of the ciliate Epistylis spp. and the bacterium Aeromonas hydrophila. J. of Protozoolgy, 25: 551-355.
Hus, T.C., W.P. Waltman and E.B.Shotts (1981): Carrelation of extracellular enzymatic activity and biochemical charasteristic with regard to virulence of Aeromonas hydrophila. Develop. Biol. Standard. 49: 101-111.
Kirov, S.M. Hui,D.S. and Hayward (1993): Milk as a potential source of Aeromonas gastrointestinal infection. J. Food Protection 56: 306-312.
Koneman,E.W.Allen,S.D.; Dowell, V.R. and Sommers,H.M. (1983): Color Atlas and rextbook of diagnostic Microbiology. 2nd Ed.,1.B. Lippincott Company, NewYork, London.
Krovacek, K.; Pasquate, V.; Baloda, S.Sporano,V.; Conte,M.Dumontet,S. (1994): Comparison of putative virulence facyors in Aeromonas hydrophila strains isolated from the marine enviroment and human diarrhea cases in sothers Italy, Appl. Environ.Microbiol., 6o (4): 1379-1382.
Kutkat, M.A.; Nagwa, S.Ata and Nagwa, S. Rabie (2001): Studies on pathogenicity of Aeromonas hydrophila in rabbit. J. Egypt. Vet. Med. Assoc. 61, 6, 2001.
MacCracken, A.W.and Barkley, R.(1972): Isolation of Aeromonas species from clinical sources. J. Clin. Pathol., 25: 970, 1972.
Mascher, E.F. ; Reinther,F.F. Steinzner, D.; Lamberger,B.. (1988): Aermonas species in a municipal water supply of a center European City : Biotyping of strains and detection toxin. Zbi. Bakt. Hyg.b, 186: 333-337.
Molero, X.; Bartolome, R.M.; Vinuesa,T. Guarner, L.Accarino, A.; Cassellas, F. Garcia, R. (1989): Acute gastroenteritis due to vibrio parahaaemolyticus in Spain. Med. Clin. Bare.Jan, 14,92 (1) 1-4.
Neves, M.S.; Nunes,M.P. and Mithomem,A.M. (1994): Aeromonas species exhibit aggregative adherence to Mep. 2 cells, J.Clin.Microb.32: 1130-1131.
Okewole,P.A.; Odeyemi,P.S.; Irokanulo,E.A.; Oyetunde, L.L. and Chine, J.C. (1989): Cholangiohepatitis and biliary fibrosis in an adult rabbit with Aeromonas hydrophila infection. Bull. Anim. Hlth. Rod. Africa 37: 395-396.
Oxoid Manual (1982) : The Oxoid manual of culture media, ingredients and other laboratory services 5th Ed. Oxoid Limit
Palumbo, A.S.; Marino,C.W.; Williams, A.C.; Buchanan, R.L.and Thraoer,D.W. (1985): Starch ampicillin agar for the quantitative detection of Aeromonas hydrophila. Appl. Environ. Microbiol, Oct. 50: 1027-1030.
Paniagua, C.; Areguello-Villares, J.L.; Arias, M.A. and herreros, H. (1998): A. hydrophila associated with a server outbreak of infection in farmed rabbits. Zentralblatt fur hygiene und Unweltmed.,. 201, 423-436.
Popoff A. M. (1984). Genus II Aeromonas. In Bergey's Manual of Systematic Bacteriology, Vol. I, ed. N.R. Krieg e J.G. Holt,
Popoff, A.M. and M. Veron (1976): A taxonomic study of the Aeromonas hydrophila Punctata group. J.G,en. Microbiol 94:11-22.
Rippey,S.R. and Cabelli,V.J. (1979): Membrane filter procedure for enumeration of Aeromonas hydrophila in fresh water. Applied and Enviromental Microbioloigy July, P. 108-113.
Shotts, E.B. and Bullock.,G.L. (1975): Bacterial disease of Fishes: Diagnostic procedures for Gram-negative pathogens. J. Fish Res. Board Can. 32, 1243-1247.
Shotts, E.B. and Rimler, R. (1973) : Medium for isolation of Aeromonas hydrophila. Appl. Microbiol. 26, 550-553.
Sohair, Z.H. and Eman,k.E.A. (2002): Occurrence of Yersina enterocolitica and Aeromonas hydrophila in pasteurized milk in Sohag City
Soliman, K.M. (1988): The pathogenesis of Aeromonas hydrophila isolates in fish with special Emphasis on their control. Thesis Ph.D Fac. Vet. Med. Alex. Univ.
Stokoph,, M.K. (1993): Fish Medicine “ Special Medicine” W.B. Saunders Co.
Toranzo A., A.M.,Baya, B.S.,Roberson, J.L. Barja (1986): Evalution of different assay systems for identification of environmental Aeromonas hydrophila strains. Appl. Environ. Microbiol. 51: 652-656.
Von.G.A. and Zinterhofer, L. (1970): The detection of Aeromonas hydrophila in stool specimens. Hlth. Lab.Sci 7: 12

Files

Share

How to cite

Statistics