SOME STUDIES ON METACERCARIAL INFECTION IN OREOCHROMIS NILOTICUS IN ASSIUT GOVERNORATE AND THEIR ROLE IN TRANSMISSION OF SOME TREMATODES TO DOGS بعض الدراسات على الميتاسرکاريا المتحوصلة فى أسماک البلطى فى محافظة أسيوط والدور الذى تلعبه تلک الأسماک فى نقل بعض الديدان الورقية للکلاب

Document Type : Original Article

Abstract

ABSTRACT:




A total number of 400 Oreochromis niloticus (Tilapia nilotica) were investigated for parasitological studies on various encysted metacercariae of digenetic trematodes in different body parts of the examined fish specimens in Assiut Governorate. The total prevalence of infected Oreochromis niloticus with different encysted metacercariae was 84.75%. They were differentiated into two types: The first type was microscopic encysted metacercariae, their prevalence rate was 78.25%. They were distributed between muscle fibers of the trunk, tail and head regions. The second one was clinostomatid metacercariae, their prevalence rate was 62.25%. The branchial chamber was the most common habitat of infection where their infection rate was 47.5% followed by kidneys 13.25% then skin 1.5%. The detected clinostomatid metacercariae were differentiated into four species: Clinostomum phalacrocoracis, Clinostomum tilapiae, Euclinostomum ardeolae and Euclinostomum heterostomum. Microscopic encysted metacercariae were differentiated into Prohemistomum vivax and Haplorchis spp. The incidence, distribution and intensity of the encysted metacercariae in different regions of the examined fish were studied. Experimental infection was carried out to confirm the identification of different kinds of microscopic encysted metacercariae by feeding parasite free puppies with different encysted metacercariae collected from the muscles of Oreochromis niloticus. Two types of adult digenetic trematodes were recovered from the intestinal mucosa (5-7 days post infection): The first type is Prohemistomum vivax and the second type is Haplorchis yokogawai.




أجريت هذه الدراسة على عدد 400 سمکة من أسماک البلطى النيلى ذات أحجام مختلفة فى محافظة أسيوط وذلک للتعرف على الأنواع المختلفة للأطوار اليرقية المتحوصلة لديدان التريماتودا. بلغت نسبة الإصابة الکلية بالأطوار اليرقية المتحوصلة 84.75% حيث تم تصنيفها إلى نوعين هما الحويصلات المجهرية وحويصلات الکلينوستوماتيد. بلغت نسبة الإصابة بالحويصلات المجهرية 78.25% وکانت منتشرة فى عضلات الجذع والذيل والرأس بينما بلغت نسبة الإصابة بحويصلات الکلينوستوماتيد 62.25%، وکان التجويف الخيشومى أکثر الأماکن تعرضاً للإصابة (47.5%) ثم تليه منطقة الکلى 13.25% بينما بلغت نسبة الإصابة فى الجلد 1.5% تم تصنيف ميتاسرکاريا الکلينوستوماتيد فى أسماک البلطى النيلى إلى أربعة أنواع، وهى کلينوستومم فالاکروکوراسز، کلينوستومم تيلابيا، إيوکلينوستومم أرديولا وإيوکلينوستومم هيتيروستومم وکذلک تم التعرف على نوعين من الميتاسرکاريا المجهرية وهى ميتاسرکاريا فيفاکس وميتاسرکاريا من نوع هابلورکس من النسيج العضلى لمنطقة الجذع والذيل والرأس. تم تحديد نسبة إصابة کل نوع من الحويصلات المختلفة وتسجيل کثافة وجودها فى أعضاء الجسم المختلفة. وقد أجريت دراسة تجريبية لمعرفة أنواع الميتاسرکاريا المجهرية المختلفة، وذلک بعدوى الکلاب الصغيرة الخالية من الطفيليات معملياً بإطعامها لحوم أسماک البلطى النيلى المصابة، وتم التعرف على نوعين مختلفين من الديدان البالغة، والتى تنتمى لعائلة الديدان الورقية هما: بروهيمستومم فيفاکس، هابلورکس يوکا جاواى عُزلت هذه الديدان من الغشاء المخاطى المبطن لأمعاء الکلاب بعد 5-7 أيام من العدوى التجريبية.

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