REFERENCES 
Amira, El-Sayed Lamey; Ammar, A. M.; 
Zaki, E. R.; Norhan, K.; Badea S. M. 
and Refai, M. K. (2013): Virulence 
factors of Escherichia coli isolated from 
recurrent cases of clinical and subclinical mastitis in buffaloes. International J. 
of Microbiological Research, 4 (1): 86-
94. 
Berkhoff, H. A. and Vinal, C. A. (1986):
Congo red medium to distinguish between invasive and noninvasive Escherichia coli pathogenic for poultry. 
Avian Diseases, 30(1): 117-121. 
Beutin, L.; Krause, G.; Zimmermann, S.; 
Kaulfuss, S. and Gleier, K. (2004): 
Characterization of Shiga toxinproducing Escherichia coli strains isolated from human patients in Germany 
over a 3-year period. J. Clin. Microbiol. 
42:1099-1108. 
Beutin, L.; Montenegro, M. A.; Orskov, I.; 
Orskov, F.; Prada, J.; Zimmermann, 
S. and Stephan, R. (1989): Close association of verotoxin (Shiga like toxin) 
production with enterohaemolysin production in strains of E. coli. J. Clin. Microbiol. 27(11):2559-2564. 
Blum, S. E. and Leitner, G. (2013): Genotyping and virulence factors assessment 
of bovine mastitis Escherichia coli. Vet. 
Microbiol., 163(3-4):305-312.
Bradley, A.J. and Green, M.J. (2001): Aetiology of clinical mastitis in six Somerest dairy herds. Vet. Rec., 148(22):683-
686. 
Breen, J. E.; Green, M. J. and Bradley, A. 
J. (2009): Quarter and cow risk factors 
associated with the occurrence of clinical mastitis in dairy cows in the United 
Kingdom. J Dairy Sci., 92(6): 2551–
2561. 
Burvenich, C.; Van Merris, V.; Mehrzad, 
J.; Diez-Fraile, A. and Duchateau, L. 
(2003): Severity of E. coli mastitis is 
mainly determined by cow factors. Vet. 
Res., 34(5):521- 564. 
Dalia, M. Mohsen and Amany, N. Dapgh 
(2007): Detection of gene sequence of
E. coli toxins isolated from mastitic buffalo and cows milk. Vet. Med. J. Giza, 
55(2): 451-467. 
DaRocha, A.C.; DaSilva, A.B.; DeBrito, 
A.B.; Moraes, H. L.; Pontes, A.P.; Ce, 
M.C.; DoNascimento, V.P. and Salle, 
C.T. (2002): Virulence factors of avian 
pathogenic E. coli isolated from broilers 
from the South of Brazil. Avian Dis., 
46(3):749-753. 
El-Khodery, S. A. and Osman, S. A. (2008): 
Acute coliform mastitis in buffaloes 
(Bubalus bubalis): Clinical findings and 
treatment outcomes. Trop. Animal 
Health and Production, 40(2): 93-99. 
El-Mahrouki, A. M.; Nevine, M. Sobhy and 
Aggour, M. G. (2006): Detection of 
coliform mastitis in cattle with special 
reference to molecular characterization 
of enterotoxigenic E. coli using polymerase chain reaction (PCR). J. Egypt. 
Vet. Med. Ass., 66(1): 47-58. 
Ericsson, U. H.; Lindberg, A.; Persson, W. 
K.; Ekman, T.; Artursson, 
K.;Nilsson-Ost M. and Bengtsson, B. 
(2009): Microbial aetiology of 
acute clinical mastitis and agent-specific 
risk factors. Vet. Microbiol., 137(1-
2):90-97.
Fagan, P.; Hornitzky, M.; Bettelheim, K. 
and Djordjevic, S. (1999): Detection of 
Shiga-like toxin (stx1 and stx2), intimin 
(eaeA), and enterohemorrhagic E. coli
(EHEC) hemolysin (EHEC hlyA) genes 
in animal feces by multiplex PCR. 
Appl. Environ. Microbiol. 65:868-872. 
Fernandes, JB. C.; Zanardo, L. G.; Galvão, 
N.N.; Carvalho, I. A.; Nero, L. A. and 
Moreira, M.A. S. (2011): Escherichia 
coli from clinical mastitis: serotypes 
and virulence factors. J. of Vet. Diagnostic Investigation, 23(6) 1146–1152. 
Gad EL-Said, W. A.; El-Jakee, J. K.; Xandel, M. M. and Mona, A. ElShabrawy (2005): Presence of E. coli 
O157:H7 in raw milk and meat samples. 
J. Egypt Vet. Med. Ass., 65(3): 341-
350. 
Galal, H. M.; Hakim, A. S. and Sohad, M. 
D. (2013): Phenotypic and virulence genes screening of Escherichia coli 
                                                                                                                strains isolated from different sources in 
delta Egypt. Life Science Journal, 
10(2):352-361. 
Güler, L. and Gündüz, K. (2007): Virulence 
properties of Escherichia coli isolated 
from clinical bovine mastitis. Turk. J. 
Vet. Anim. Sci., 31(5): 361-366. 
Gyles, C. L. (1993): Escherichia coli. In: 
Gyles, C. L., Thoen, C.O. (Eds.). 
Pathogenesis of bacterial infections in 
animals. Iowa State University Press, 
Ames, Iowa, pp. 164-187. 
Hogan, J. S and Smith, K. L. (2003): Coliform mastitis. Vet. Res., 34:507-519. 
John, M.; Jacques, M. and Lariviere, S.
(1989): Pathogenicity of E.coli O115:K 
V165 strains isolated from pigs with diarrohea. Am. J. Vet. Res., 50(1): 1029-
1036. 
Kaipainen, T.; Pohjanvitra, T.; Sphigel, N. 
Y.; Shwimmer, A.; Pyolara, S. and 
Pelkonen, S. (2002): Virulence factors 
of E. coli isolated from bovine clinical 
mastitis. Vet. Microbiol., 85(1):37-46.
Kalmus, P.; Aasmäe, B.; Kärssin, A.; Orro, 
T. and Kask, K. (2011): Udder pathogens and their resistance to antimicrobial agents in dairy cows in Estonia. 
Acta Vet Scand. 53: 4. 
Kaper, J. B.; Nataro, J. P. and Mobley, H. 
L. (2004): Pathogenic E. coli. Nat. 
Rev. Microbiol., 2:123–140. 
Kok, T.; Worswich, D. and Gowans, E. 
(1996): Some serological techniques for 
microbial and viral infections. In Practical Medical Microbiology (Collee, J.; 
Fraser, A.; Marmion, B. and Simmons, 
A., eds.) 14th ed., Edinburgh, Churchill 
Livingstone, UK. 
Koneman, E. W.; Allen, S. D.; Dowell V. R. 
and Sommer H. M. (1983): The Enterobacteriaceae, in: Color Atlas and 
Textbook of Diagnostic Microbiology, 
J.B. Lippincott Company, New York, 
USA, pp. 57– 124. 
Lehtolainen, T. (2004): E. coli mastitis: Bacterial factors and host response, Ph. D. 
thesis Department of clinical veterinary 
sciences, Faculty of veterinary Medicine, Helsinki, University, Finland. 
Lira, W. M.; Macedo, C. and Marin, J. M. 
(2004): The incidence of Shiga toxinproducing Escherichia coli in cattle 
with mastitis in Brazil. J.of Applied Microbiology, 9: 861–866. 
Longo, F.; Salat, O. and Gool, V.F. (2001): 
Incidence of clinical mastitis in French 
dairy herds: epidemiological data and 
economic costs. Folia Veterinaria, 45: 
45-46. 
McKillip, J. and Drake, M. (2000): Molecular beacon polymerase chain reaction 
detection of Escherichia coli O157:H7 
in milk. J. Food Protect. 63: 855-859.
Momtaz, H.; Dehkordi, F. S.; Taktaz, T.; 
Rezvani, A. and Yarali, S. (2012): 
Shiga toxin-producing Escherichia coli
isolated from bovine mastitic milk: Serogroups, virulence factors, and antibiotic resistance properties. The Scientific 
World Journal, Article ID 618709, 9 
pages. 
Mosherf, B. E. (2004): Studies on microbial 
causes of mastitis in buffaloes, Ph. D. 
thesis (Microbiology), Fac. Vet. Med. 
Cairo Univ. 
Moussa, I. M.; Mostafa, M. and Mohamed, 
K. F. (2006): Determination of phylogenetic relationships among E. coli
isolates recovered from bovine fecal 
and milk samples. J. Egypt. Vet. Med. 
Ass., 66: 7-25. 
Murinda, S.E.; Nquyen, L.T.; Landers, 
T.L.; Draughon, F.A.; Mathew, A.G.; 
Hogan, J.S.; Smith, K.L.; Hancock, 
D.D. and Oliver, S.P. (2004): Comparison of Escherichia coli isolates 
from humans, food and farm and companion animals for presence of Shiga 
toxin-producing E. coli virulence markers. Foodborne Pathogens and Disease, 
1(3): 178-184. 
Oliver, S.P. (1988): Frequency of isolation of 
environmental mastitis-causing pathogens and incidence of new intramammary infection during the non-lactating 
period. American Journal of Veterinary 
Research, 49(11): 1789-1793 .
                                                                                                                Panigrahy, B. and Yushen, L. (1990): Differentiation of pathogenic and non 
pathogenic Escherichia coli isolated 
from poultry. Avian Dis., 34: 941-943.
Pass, M., Odedra, R. and Batt, R. (2000): 
Multiplex PCR for identification of Escherichia coli virulence genes. J. Clin. 
Microbiol. 38:2001-2004. 
Quesnell, R. R.; Klaessig, S.; Watts, J. L. 
and Schukken, Y. H. (2012): Bovine 
intramammary Escherichia coli challenge infections in late gestation demonstrate a dominant anti-inflammatory 
immunological response. J. Dairy Sci., 
95(1):117-126. 
Quinn, P. J.; Carter, M. E.; Markey, B. and 
Carter, G. R. (2004): Clinical veterinary microbiology. 6th ed., Mosby, Edinburgh, London, New York, Philadelphia, St. Louis, Sydney, Toronto. 
Rangel, P. and Marin, J. M. (2009): Analysis of Escherichia coli isolated from bovine mastitic milk. Pesq. Vet. Bras. 
29(5):363-368. 
Salman, A. M. (1999): Serological and bacteriological studies on Escherichia coli in 
chicken. Ph. D. Thesis (Microbiology), 
Fac. Vet. Med., Cairo Univ. 
Schukken, Y.H,; Bennett, 
G.J.; Zurakowski, M.J.; Sharkey, 
H.L.; Rauch, B.J.; Thomas, M.J.; 
Ceglowski, B.; Saltman, 
R.L.; Belomestnykh, N. and Zadoks 
R.N. (2011): Randomized clinical trial 
to evaluate the efficacy of a 5-day 
ceftiofur hydrochloride intramammary 
treatment on non severe gram-negative 
clinical mastitis. J. Dairy 
Sci., 94(12):6203-6215. 
Sereny, B. (1955): Experimental shigella 
keratoconjunctivitis. Acta Microbiol. 
Acad. Sci. Hung., 2: 293-296. 
Sharma, K. K.; Soni, S. S. and Meharchandani, S. (2006): Congo red dye test as 
an indicator test for detection of invasive bovine Escherichia coli – short 
communication. Veterinarski Arhiv, 
76(4):363-366.
Taylor, P. w. (1983): Bacteriocidal and bacteriolytic activity of serum against Gram 
negative bacteria. Microbial. Rev., 47: 
46-83. 
Timmis, K. N. (1979): Plasmid gene that 
specifies resistance to the bactericidal 
activity of serum. In Plasmids of medical, environmental and commercial importance. Edited by K. N. Timmis & 
Puhler, A. (Elsevier, North Holland 
Biomedical Press, New York, 145-153. 
Wenz, J. R.; Barrington, G. M.; Garry, F. 
B.; Ellis, R. P. and Magnuson, R. J. 
(2006): E. coli isolates, serotypes, genotypes and virulence genes and clinical 
coliform mastitis severity. Journal of 
Dairy Science, 89(9): 3408-3412. 
Zaki, E.R.; Riad, E. M. and Sobhy, N. M. 
(2004): Correlation between E. coli serotypes isolated from buffalo mastitic 
milk with different virulence patterns. J. 
Egypt Vet. Med. Assoc., 64: 53-63. 
Zschock, M.; Hamann, H. P.; Kloppert, B. 
and Wolter, W. (2000): Shiga-toxin 
producing E.coli in faeces of healthy 
dairy cows, sheep and goats: prevalence 
and virulence properties. Lett. Appl. 
Microbiol. 31:203-208.