A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates إسهام ميکروبات الکوليفورم في إحداث التهاب الضرع في الأبقار، مع الإشارة إلي التصنيف السيرولوجي وتحديد عوامل الضراوة لعترات الميکروب القولوني

Document Type : Original Article

Abstract

ABSTRACT
Escherichia coli (E. coli) is the predominant coliform species causing intramammary infec￾tions. Where in the present study, E. coli isolates were 18 strains (17.82%) followed by Enterobacter
aerogenes 3 strains (2.97%) and Klebsiella pneumoniae one strain (0.99%) from 101 clinical mastitic
milk samples of cows. Eighteen E. coli isolates were serotyped to nine different serogroups; O111:H4
(3), O127:H6 (3), O26 (2), O126 (2), O119:H6 (1), O114:H21 (1), O55:H7 (1), O44:H18 (1), O124 (1)
and (3) untyped. Virulence tests were performed on the 18 isolated E. coli, it was found that 15 isolates
(83.3%) were serum resistant, 13 isolates (72.2%) had Congo Red binding activity, 6 isolates (33.3%)
were invasive and one isolate (5.6%) had haemolytic activity. PCR was applied to detect the presence
of Shiga like toxin producing E. coli (stx1 and stx2 genes) on the nine different strains (one strain for
each serogroup), where stx1 and stx2 were found in 8 (88.9%) and 4 (44.4%) of the nine examined
strains, respectively. While stx1 and stx2 genes were found together in 3 strains (33.3%). Conclusions:
E. coli isolates usually posses one or more virulence factors that may help in establishment at the in￾fection site and subsequently causing clinical bovine mastitis.
الملخص العربي :
يعتبر الميکروب القولوني الأکثر شيوعاً في مجموعة ميکروبات الکوليفورم المسببة لالتهاب الضرع في
الأبقار. حيث تم عزل ١٨ عترة (١٧،٨٢%) من الميکروب القولوني يليها الأنتيروباکتر أيروجينز ٣ عترات
(٢،٩٧%) ثم ميکروب الکيلبيسيلا نيموني عترة واحدة (٠،٩٩%) من ١٠١ عينة لبن لأبقار إصابة بالتهاب
الضرع الإکلينيکي. تم أجراء الفحص السيرلوجي لعترات الميکروب القولوني حيث تبين أنها تنتمي إلي تسع
O111:H4(3), O127:H6(3), O26(2), O126 (2), O119:H6 (1), O114:H21 (1), هي مجموعات
الضراوة اختبارات بإجراء .تصنيفها يتم لم عترات وثلاث O55:H7 (1), O44:H18 (1) and O124 (1)
لعترات الميکروب القولوني المعزولة (١٨ عترة), وجد ١٥ عترة (٨٣،٣%) مقاومة للمصل و١٣ عترة
(٧٢،٢%) لديها القدرة علي الارتباط بصبغة الکونغو الأحمر و6 عترات (٣٣،٣%) لديها القدرة علي أحداث
التهاب ملتحمة العين في خنازير غينيا وعترة واحدة (٥،٦%) لديها المقدرة علي تحليل الدم. وباستخدام تفاعل
البلمرة المتسلسل علي تسع عترات مختلفة للميکروب القولوني (عترة واحدة لکل مجموعة), وجد ٨ عترات
(٨٨،٩%) تحتوي علي الجين ٤ stx1 و عترات (٤٤،٤ ت%) حتوي علي الجين stx2. کما أسفرت النتائج عن
ثلاث عترات (٣٣،٣%) تحتوي علي الجينين stx1 and stx2 معا. الخلاصــة: عترات الميکروب القولوني
المعزولة لديها واحد أو أکثر من عوامل الضراوة التي تمکنها من إحداث التهاب الضرع الإکلينيکي.

Keywords


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